Measurements of excision repair tracts formed during meiotic recombination in Saccharomyces cerevisiae.

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Excision repair in the yeast, Saccharomyces cerevisiae.

cdc9 mutants of yeast lack detectable DNA ligase activity at restrictive temperatures. They also appear to be more sensitive than wild-type cells to ultraviolet (u.v.) radiation and it has been assumed that this is because the CDC9 ligase is needed for the final ligation step in excision repair. The fact that single-strand breaks have been demonstrated in u.v.-irradiated cdc9 mutants has been r...

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Initiation of Ageing Process by Meiotic and Mitotic Recombination within the Ribosomal DNA Genes in Saccharomyces cerevisiae

In the budding yeast of Saccharomyces cerevisiae the tandem repeated of rDNA genes are located onchromosome XII, which is in the nucleolus. There are different types of proteins in the nucleoluskeleton,silencing proteins have got important role in nucleolus.It is shown that meiotic recombination between nonsister chromatids in the rDNA genes are stronglysuppressed, and s...

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Analysis of meiotic recombination initiation in Saccharomyces cerevisiae

Meiosis is the unique process in which diploid cells undergo two consecutive divisions to produce haploid daughter cells. It is indispensable for sexual reproduction in all eukaryotic organisms and maintains proper chromosome number through generations. An integral step in the meiotic program is genetic recombination; recombination is required for a successful reductional division. In the yeast...

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Meiotic recombination at the ends of chromosomes in Saccharomyces cerevisiae.

Meiotic reciprocal recombination (crossing over) was examined in the outermost 60-80 kb of almost all Saccharomyces cerevisiae chromosomes. These sequences included both repetitive gene-poor subtelomeric heterochromatin-like regions and their adjacent unique gene-rich euchromatin-like regions. Subtelomeric sequences underwent very little crossing over, exhibiting approximately two- to threefold...

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A test of the double-strand break repair model for meiotic recombination in Saccharomyces cerevisiae.

We tested predictions of the double-strand break repair (DSBR) model for meiotic recombination by examining the segregation patterns of small palindromic insertions, which frequently escape mismatch repair when in heteroduplex DNA. The palindromes flanked a well characterized DSB site at the ARG4 locus. The "canonical" DSBR model, in which only 5' ends are degraded and resolution of the four-st...

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ژورنال

عنوان ژورنال: Molecular and Cellular Biology

سال: 1992

ISSN: 0270-7306,1098-5549

DOI: 10.1128/mcb.12.4.1805